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Resazurin, a non-fluorescent indicator dye, is converted to highly red fluorescent resorufin via reduction reactions of metabolically active cells. The amount of fluorescence produced is proportional to the number of living cells.
Alizarin Red S Staining for Osteogenesis. Alizarin Red is used to identify calcium deposits in tissue sections. Calcium forms an Alizarin Red S-calcium complex in a chelation process.
This video describes the procedure of Alizarin Red S Staining for osteogenesis. Antibody Conjugation - Biotinylation. Antibodies labeled with biotin provide the user with a tool for increasing the sensitivity of an assay by its ability to amplify a given reaction.
The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate FITC is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophores high quantum efficiency and conjugate stability.
Antibody Pair for Proximity Ligation Assay. The in situ proximity ligation assay is a powerful technology capable of detecting single protein events such as protein protein interactions e. Each detected signal is visualized as an individual fluorescent dot, these signals can be quantified counted and assigned to a specific subcellular location based on microscopy images.
Antibody Purification Affinity. Cyanogen bromide CNBr is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix. Antibody Purification Protein A Column. The coupling technique is optimized to give a high binding capacity for IgG.
This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid. Polyethylene Glycol PEG is a long chain polymer that has been approved by the Food and Drug Administration for human intravenous, oral and dermal applications. Covalent attachment of PEG PEGylation to proteins can reduce their immunogenicity, minimize proteolytic cleavage and increase their serum half-life.
PEG has also been attached to small molecules and liposomes for more selective delivery. PEG-modification of superparamagnetic iron oxide and quantum dots can improve their biocompatibility and reduce non-specific uptake.
The second part of the assay uses the Caspase-GloR to measure caspase activity. Besides the temperature logger, self-contained biological indicators that contain G. Learn how to use the biological indicator in this AbVideo. There are also physical and chemical indicators that can be used to ensure decontamination effectiveness of autoclaves. Stainless steel loggers are commonly used for monitoring temperature of autoclave.
Check out how to use it to maintain the maximum performance of your autoclave machine. There are physical, chemical, and biological indicators that can be used to ensure decontamination effectiveness of autoclaves. Bioburden Test of BSC. Biological Safety Cabinets BSC is an enclosed, ventilated laboratory workspace which provides the most effective primary containment for working with infectious agents.
The BSC is tested for bioburden to ensure the adequate protection. A blood smear gives information about the number and shape of blood cells. To perform a blood smear, two very clean slides are required. Drop a small drop of blood on one of the slides. Pull blood forward across slide. Calcium Phosphate Transfection. This method works best in cell lines that are highly transformed and adherent and this technique requires few manipulative steps and maintains high levels of reproducibility from experiment to experiment.
Cationic Polymer Transfection. The cells must be fixed and permeabilized to ensure free access of the antibody to its antigen. Fixation methods fall generally into two classes: organic solvents and cross-linking reagents. This video uses formaldehyde as a cross-linking reagent to fix cells.
CellTiter 96R AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity assays. The reagent contains a tetrazolium compound MTS. The MTS is bioreduced by cells into a colored formazan product which can be measured by the absorbance at nm.
By cellular mitochondrial dehydrogenases, WST-1 is bioreduced into formazan which can be measured at an absorbance of nm. Cell Synchronization with Nocodazole at Mitosis. Cell Synchronization is a process by which cells at different stages of the cell cycle in a culture are brought to the same phase. Nocodazole is one of anticancer drugs that synchronize cells at M-phase. It can interfere with the structure and function of microtubules in interphase and mitotic cells.
Chimera RNA Interference. Kumiko Ui-Tei at the University of Tokyo. Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation.
Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate. The auto gas analyzer is a tool to provide accurate carbon dioxide CO2 measurements in incubators. The Fyrite gas analyzer is the most widely used instrument for measuring carbon dioxide CO2 levels in incubators. It uses the Orsat method of volumetric analysis involving chemical potassium hydroxide absorption of CO2 gas.
Competent Cell Preparation. Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. Because DNA is hydrophilic, it will not normally pass through membrane. In order to make these cells readily incorporate foreign DNA, they must first be made "competent" to take up foreign DNA. After washing, unbound antibodies are removed. The more analytes in the sample, the less antibodies will be able to bind to antigens in the well.
The signal is then detected using labeled secondary antibodies and the decrease in signal is compared to a control. The major advantage of a competitive ELISA is the ability to use crude or impure samples and still selectively bind any antigen that may be present.
Diethylpyrocarbonate DEPC is used in the laboratory to inactivate the RNase enzymes from water and other laboratory utensils. It inactivates the RNases by the covalent modifications of the histidine residues.
Human umbilical vein endothelial cells HUVEC are commonly used as a laboratory model system for the physiological and pharmacological investigations. Dialysis ultrafiltration is a method to concentrate protein or other macromolecules through a membrane with defined pores. The membranes will have a molecular weight cut-off MWCO.
This is the limit of size or range of a protein that can fit through the membrane. DNA sequencing is a technique to determine the order of the nucleotide bases-adenine, guanine, cytosine, and thymine. It is indispensable for basic biological research and discovery. Dot blot is a technique can be used as a semiqualitative method for rapid screening of a large number of samples. It is for detecting, analyzing, and identifying proteins, similar to the western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane.
Electroporation applies a large electric pulse temporarily disturbs the phospholipid bilayer, allowing molecules to pass into the cell. It is usually used in molecular biology as a way of introducing some substance into a cell, such as molecular probes, drugs or pieces of coding DNA. Emulsification is a process to mix adjuvants and immunogen. Adjuvants are mixed and injected with an antigen to prevent catabolism and help increase the immune response by localizing the antigen for an extended time and attracting the appropriate cells T cells, B cells and APC to interact with it.
Ethidium Bromide EtBr is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis. It is a potent mutagen, so its hazardous properties require special safe handling and disposal procedures. Fluorescent Microscope. A fluorescent microscope is an optical microscope used to study properties of substances using the phenomena of fluorescence and phosphorescence.
Specimen can be labeled specifically with a fluorescent molecule and be illuminated with specific wavelength which is absorbed by the fluorophores.
Gelatin gel zymography in analyze the MMP is now recognized as the important process in cell migration, invasion or tissue remodeling. GST Enzymatic Digestion. GST fusion proteins allow convenient affinity purification of many proteins of interest but the GST-tag may become inconvenient in various downstream applications. GST-tagged Protein Purification.
Protein purification is a series of processes intended to isolate a single type of protein from a complex mixture. We use GST-fusion protein to purify and detect proteins of interest. The GST-fusion protein can be purified from cells via its high affinity for glutathione. As a mature diagnostic tool, regular blood test is widely adopted to evaluate anemia, leukemia, reaction to inflammation and infections, etc.
The test collects information including the number and types of different types of blood cells, the variation in the size of red blood cells, hematocrit, hemoglobin value, platelet count, mean corpuscular hemoglobin, and the average size of red blood cells.
Here we demonstrate a simple operation of hematology test for following clinical diagnosis. His-tagged Protein Purification. Proteins with histidine tag can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions.
Immunofluorescence is a technique to visualize a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye.
EZ-PCR™ Mycoplasma Detection Kit
The supplier does not provide quotations for this particular product through SelectScience. You can search for similar products in our product directory. Routine mycoplasma testing should be carried out minimally every 3 to 6 months, as well as prior to the incorporation of new cultures from outside sources. Universal: Detects a variety of mycoplasma species: M. Manufacturer Biological Industries Be the first to review this product.
AbVideo™ - Mycoplasma Detection - PCR Method (EZ-PCR Mycoplasma Test Kit)