COUNTER CURRENT IMMUNOELECTROPHORESIS PDF

The countercurrent immunoelectrophoresis CIE test was performed as a diagnostic tool for bovine babesiosis using a partially purified soluble antigen obtained from a saline extract of B. Antibodies against B. These antibodies persisted until the end of the observation period 90th day post-infection. In contrast, the 20 serum samples from a Babesia-free area were all negative by both these methods.

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Last Updated on: January 8, by Sagar Aryal. Countercurrent immunoelectrophoresis is mostly carried out with one or more of the following objectives:. Counter-current immunoelectrophoresis depends on the movement of antigen towards the anode and of antibody towards the cathode through the agar under the electric field.

The test is performed on a glass slide in agarose gel of high electro-endosmotic flow. A pair of wells is punched out where one well is filled with antigen and the other with the antibody.

Electric current is then passed through the gel. The migration of antigen and antibody is greatly facilitated under the electric field, and the line of precipitation as precipitin arcs is made visible in 30—60 minutes, which indicates a positive reaction.

Table of Contents hide. Counter Current Immunoelectrophoresis Objectives. Counter Current Immunoelectrophoresis. Share the article on:. Related Notes:. Opsonization- Definition, Mechanism, Opsonins, Examples. Endocytosis- Definition, Process and Types with Examples. Immune Booster Foods. Ouchterlony Double Immunodiffusion technique. Physiological barriers of immune system. Complement Components.

Complement- Properties and Nomenclature.

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Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.

Counter-current immunoelectrohporesis is a rapid sensitive method for detecting pneumococcal capsular antigens in sputum. A result can be obtained within 45 minutes. The optimum conditions for performing the test are given. Counter-current immunoelectrophoresis works with all the 33 pneumococcal antigens tested except type Better precipitin lines are obtained when the gel-support is acid pH than when it is alkaline pH Omniserum is as effective as group-specific sera for the identification of pneumococcal capsular antigens. The clinical importance of these findings is still being studied but our experience has shown that patients with chest infections should have effective antipneumococcal antibiotics as part of their regimen and that the laboratory diagnosis may be made quickly and accurately by counter-current immunoelectrophoresis.

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